Although some cell types such as CHO cells are well adapted to use in bioreactors, many other cells, including stem cells, are too fragile to be grown to high density in bioreactors. These cells need a microenvironment in which they can grow and which protects them from the sheer forces associated with stirred or perfused bioreactors.
Cell-in-a-Box® provides such a microenvironment for fragile cells. The capsules can be vigourously stirred inside a bioreactor and the cells inside are well protected from the sheer forces.
Further, since the capsules are porous, larger molecules are retained within the capsule and only the desired products are released into the media of the bioreactor.
In cases where the aim is to harvest the actual encapsulated cells, we offer a proprietory reagent which allows the capsules to be disintegrated without affecting the cells inside, allowing an easy harvest of free cells after bioreactor growth.
To demonstrate the use of Cell-in-a-Box® technology for protection of stem cells from sheer forces, we have for instance shown that non-encapsulated stem cells are destroyed after short periods of vortexing, whilst encapsulated cells survive easily.
Advantages of encapsulating cells for use in bioreactors:
- Protects fragile cells from sheer forces
- Provides a microenvironment for growth
- High density growth possible
- Capsules act as a pre-filtration device for produced factors
- Cells can be released from filled capsules using a proprietary non-toxic reagent